SciELO - Scientific Electronic Library Online

vol.12 número1Un estudio teórico AIM (Atoms in Molecules) con el fin de evidenciar la existencia de puentes de hidrógeno en varios derivados tiazólicosEvidencia ultraestructural del nucléolo de Entamoeba histolytica índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados

  • No hay artículos similaresSimilares en SciELO


TIP. Revista especializada en ciencias químico-biológicas

versión impresa ISSN 1405-888X


SANZ-OCHOTORENA, Ana et al. Ultraestructura de la gónada del macho de dos subespecies de Bufo longinasus. TIP [online]. 2009, vol.12, n.1, pp.27-33. ISSN 1405-888X.

Bufo longinasus is classified as endangered in the IUCN Red List of Threatened Species. There is very little knowledge about this species as well as the possibility of the existence of subspecies, so the results of this research may help to clarify systematic aspects. The main goal of the study is to describe the male gonad of Bufo longinasus by Transmission Electron Microscopy. Four male toads of B.longinasus longinasus and B. longinasus cajalbanensis in reproductive period were collected in Pinar del Rio Province. Once the specimens were in the laboratory, they were anesthetized with ether. Gonads were observed in situ, and then dissected and placed in transmission electron fixatives. The results show that the male gonad has Bidder´s organ just above the testicles. The testicle has a highly pigmented tunica albuginea. Oocytes from the ovary and Bidder´s organ were morphologically identical. With respect to proliferation and cell differentiation, it appears that the cell types (spermatogonia, spermatocytes, early, middle and late spermatids, and sperm) have a cystic organization, that is, they form groups of cells associated to Sertolli cells. The sperm cells are characterized by an extraordinary nuclear compactness and the presence of an undulating membrane.

Palabras llave : Bufo longinasus; sperm; gonads; Bidder’s organ; pigments.

        · resumen en Español     · texto en Español     · Español ( pdf )