Resumen

HAYGERT-LENCINA, Kelen; ANTONIO-BISOGNIN, Dilson; KIELSE, Paula  y  PIMENTEL, Nathalia. Rooting and acclimatization of Apuleia leiocarpa plantlets. Agrociencia [online]. 2017, vol.51, n.8, pp.909-920. ISSN 2521-9766.

Apuleia leiocarpa is an endangered tree species with high quality timber. Propagation by seed is difficult due to irregular fruiting and water-impermeable tegument, so asexual propagation is a promising alternative. This study aimed to evaluate in vitro and ex vitro rooting of A. leiocarpa and acclimatization of micropropagated plantlets. The hypothesis was that type of explant, indolebutyric acid (IBA) concentration and substrate influence the induction of adventitious roots in apuleia. For in vitro rooting, nodal segments and micro-cuttings were grown in Wood Plant Medium (WPM) with 0, 4.9, 9.8, 14.7 and 19.6 μM IBA. Compositions of commercial substrate + vermiculite + coarse sand and commercial substrate + vermiculite, in equal proportions, were tested for acclimatization. For ex vitro rooting, nodal segments and micro-cuttings were treated with 0 and 4920 μM IBA for 10 s and grown in equal proportions of: commercial substrate + vermiculite + coarse sand, commercial substrate + vermiculite, and commercial substrate + coarse sand. Nodal segments presented greater rooting in vitro than micro-cuttings without need for IBA supplementation in the culture medium. Substrate composition did not affect survival or growth of in vitro rooted plantlets during acclimatization. The best ex vitro rooting was found in nodal segments and explants treated with 4920 μM IBA and grown in commercial substrate + vermiculite + coarse sand. Ex vitro rooting is a promising technique for producing apuleia plantlets; however, adjustments to environmental conditions in both ex vitro rooting and acclimatization are necessary in order to maximize survival of micropropagated A. leiocarpa plantlets.

Palabras llave : Apuleia leiocarpa; indolebutyric acid; micropropagation; micro-cuttings; substrate composition.

        · resumen en Español     · texto en Español | Inglés     · Español ( pdf ) | Inglés ( pdf )