Resumen

GARZA-LOPEZ, Paul M.; KONIGSBERG, Mina; SAUCEDO-CASTANEDA, Gerardo  y  LOERA, Octavio. Differential profiles of Beauveria bassiana (Bals.-Criv.) Vuill. as a response to CO₂: production of conidia and amylases. Agrociencia [online]. 2011, vol.45, n.7, pp.761-770. ISSN 2521-9766.

Conidia production of entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill. is performed in a solid state culture (SSC) using agricultural products and their residues, such as rice (Oryza sativa) and sugarcane (Saccharum ojjicinarum) bagasse, where CO₂ can be accumulated as a product of metabolism. In this study rice (Oryza sativa var. japonica) was used as substrate using two atmospheric compositions: normal air and other enriched with CO₂ (5 %), through exchanges every 12 or 24 h. The effect of these treatments was determined on the conidial production and amylase enzymes by B. bassiana, using a completely randomized design and analysis of variance using Tukey-Kramer test (p< 0.05). Maximum conidia production per g of initial dry substrate (gssi) was obtained with the normal atmosphere (1.14×10⁹ conidia gssi^-1), while with 5 % of CO₂ the conidia production decreased up to 85 % (1.8×10⁸ conidia gssi^-1). However, in this latter treatment amylase activity increased three times (16.58 UI gssi^-1) with respect to the normal atmosphere (5.41 UI gssi^-1). These results are important for the conidia and enzymes production by fungi in SSC, with the recommendation to modify the concentration of CO₂, as it is required to favor the type of product.

Palabras llave : Beauveria bassiana; Oryza sativa; carbon dioxide; solid medium culture; yield of conidia; enzymatic activity.

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