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Agrociencia

versión On-line ISSN 2521-9766versión impresa ISSN 1405-3195

Resumen

GASCA-GONZALEZ, Ma. Rosario et al. Study on the transcriptome in Capsicum chinense Jacq. resistant to pepper huasteco yellow vein virus (PHYVV). Agrociencia [online]. 2008, vol.42, n.1, pp.107-117. ISSN 2521-9766.

In order to enrich the knowledge of transcriptome in incompatible interaction between PHYVV and Havana chili pepper plants (Capsicum chinense Jacq.) from the INIFAP collection denominated BG-3821, the profile of gene expression was studied. These studies are essential for the knowledge at molecular level of signaling mechanisms for mutual recognition and, in its case, the defense response of the host to the pathogen, which may contribute to the design of new crop protection strategies. A differential expression model was used in plants infected by PHYVV and subtractive hybridization methodology by suppression (SSH), in order to obtain a library of 99 gene fragments (EST), whose expression was specifically induced. The sequence of the obtained EST's showed that the genes expressed in this interaction may be grouped in the following categories: a) possible resistance genes; b) genes involved in genetic regulation routes; c) genes with unknown function. By Northern-type analysis the differential expression of one EST (R100 clone) was confirmed, selected on the basis of its similarity of sequence with genes playing a potential role in resistance mechanisms. With the results of this research, first evidence of genes is presented, that possibly may be involved in recognition and signaling routes in C. chinense in response to PHYVV.

Palabras llave : Capsicum chinense; geminivirus; transcriptome; PHYVV.

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