Abstract

CAVAZOS-GALINDO, Jaime Manuel et al. Clonal propagation of two adult grapevines cultivars (Vitis vinifera L.) for in vitro conservation. Polibotánica [online]. 2018, n.45, pp.181-190. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.45.13.

The in vitro propagation of two cultivars of grapevine (Vitis vinifera L.) Cabernet Sauvignon and Merlot, began using axillary buds and meristems as explants source, these were established in Murashige and Soog (MS) medium plus 6-benzylamino purine (BAP) at 0.5, 1.0 and 1.5 mg L-1. The variables evaluated were number and length of buds. The greatest response was provided with 1.0 mg L-1 of BAP in both explants. For multiplication and shoot rooting, explants were subcultured on MS medium at 50 and 100% and added with 0.5 and 1.0 mg L-1, 0.5 mg L-1 indolebutyric acid (IBA) and vitamins. The best response for bud numbers was 6.37, while for shoot length 2.72 cm, both in the MS medium at 50% with 1.0 mg L-1 of BAP. The highest value obtained for number and length of roots was in MS medium at 50% with 0.5 mg L-1 BAP that obtained 100% rooting in both cultivars.

Keywords : Micropropagation; Cabernet Sauvignon; Merlot.

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