Abstract

GARZA-PADRON, R.A. et al. Amoebicid and antioxidant activity and phytochemical profile of methanolic extracts of callus and stem of the cactus Astrophytum myriostigma. Polibotánica [online]. 2010, n.30, pp.111-121. ISSN 1405-2768.

Methanolic extracts were obtained from both in vitro and ex vitro tissues of Astrophytum myriostigma to develop a phytochemical profile, probe antioxidant activity (DPPH 2, 2-diphenyl-1-picryl-hydrazyl) and evaluate amoebicid activity against Entamoeba histolytica HM1-IMSS. The phytochemical profile results indicate the presence of different secondary metabolites in callus and stem; whereas the callus has phenolic oxidrils and flavonoids, these are not present in the stem, which may cause the difference in the antioxidant activity between callus with an IC₅₀ of 199.19 μg/mL and stem with 3961.39 μg/mL. The amoebicid activity was satisfactory with an IC₅₀ of <100μg/mL for both extracts. The presence of these metabolites in callus of Astrophytum myriostigma is probably due to conditions and nutrients present in media.

Keywords : cactus; tissue culture; methanolic extracts; Astrophytum myriostigma; biological activity.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )