Revista Chapingo. Serie horticultura
versão impressa ISSN 1027-152X
FLORES-ESCOBAR, Georgina; GIL-VASQUEZ, Isaías; COLINAS-LEON, María Teresa e MATA-ROSAS, Martín. In vitro propagation of Brassia verrucosa (Bateman ex Lindl.) orchid. Rev. Chapingo Ser.Hortic [online]. 2011, vol.17, n.1, pp. 5-8. ISSN 1027-152X.
Brassia verrucosa Bateman ex- Lindl was propagated in vitro to determinate the best conditions of culture for seed germination and seedling development, to facilitate the design of an initial strategy for its conservation and rescue. Seeds were germinated in Murashige and Skoog (MS) medium, and seedling were grown in MS (T1), MS supplemented with organic extracts, coconut water, peptone and active charcoal (T2) medium and MS supplemented with organics extracts, coconut water, peptone and gibberellic acid (T3). Germination was 56.70 %, initiating six days after sowing. The variables number of buds, leaf width and root length were affected by the treatments, but not leaf length, root number or seedling height. The Tukey analysis showed significant differences for all the variables with the exception of leaf width and root length. T2 showed the highest values for the variables number of buds, leaf length, root number and seedling height. Germination and development of the plants are the beginning of a strategy for conservation and rescue of the species.
Palavras-chave : Germination; rescue; activate charcoal; growth.