Abstract

SOSA-RODRIGUEZ, F. M. et al. Propagación in vitro de Heliconia standley Macbride en Cuba. Rev. Chapingo Ser.Hortic [online]. 2009, vol.15, n.spe, pp.17-23. ISSN 2007-4034.

A protocol was applied that allowed the micropropagation of the ornamental species of Heliconia standleyi Macbride. Different variants of disinfestations of plants, culture medium and explants handling were studied during the different stages of the process and finally their acclimatization. The cultivar can be propagated in liquid medium using a disinfestations with sodium hypochlorite (NaOCl) 2% during 20 min, cultured medium supplemented with 6-BAP (2.0 mg·litro ^-1), AIA (0.65 y 1.3 mg·litro^-1) and explants had a size bigger than 1.0 cm in semi liquid medium. The multiplication coefficient after subculture was 4.6 explants/meristems. Optimum results in the roots production for addition in medium of AIA 1.3 mg·litro^-1 were obtained. The acclimatization was satisfactory in greenhouse always the size of plants were between 3 - 5 cm and until 45 days in controlled conditions.

Keywords : micropropagation; acclimatization.

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