Revista Chapingo. Serie horticultura
Print version ISSN 1027-152X
CUIRIS-PEREZ, H. et al. Genetic variability within mexican race avocado (Persea americana Mill.) germplasm collections determined by ISSRs. Rev. Chapingo Ser.Hortic [online]. 2009, vol.15, n.2, pp. 169-175. ISSN 1027-152X.
The present study was undertaken in order to asses the existing genetic diversity within a germplasm collection of avocado (Persea americana Mill.) kept at the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) Campo Experimental Uruapan (CEFAP-Uruapan). The parental relationship among 77 accessions (231 plants) of the Mexican race was investigated by using seven Inter Simple Sequence Repeat microsatellites (ISSRs). A total of 154 loci were detected. Percentage polymorphism ranged from 82.3 to 95.4, with number of bands ranging from 17 to 25 within accessions. Genetic similarity analysis revealed formation of two major groups, one with eleven subgroups and the other one with three subgroups. Genetic similarity was highest between accession 237 (Atlixco, Puebla) and accession XTC01 (Uruapan, Michoacan), while accessions 532 (Atlixco, Puebla) and 369 (Chilchota, Michoacan) were the most dissimilar ones. None of the accessions were found to be duplicates. In general, the present study demonstrated the usefulness of ISSRs analysis for determination of genetic diversity in avocado.
Keywords : microsatellites; germplasm; genetic similarity.