Revista Chapingo. Serie horticultura
versão impressa ISSN 1027-152X
HERMOSILLO-CERECERES, M. A. et al. Genetic relationship between experimental long chilaca-type chili pepper materials and commercial varieties. Rev. Chapingo Ser.Hortic [online]. 2008, vol.14, n.3, pp. 301-307. ISSN 1027-152X.
The use of foreign varieties of chilaca-type chili peppers has had negative consequences: high production costs and limited acceptance primarily because of a lack of a pleasant spicy flavor. To improve these characteristics, a plant breeding program was developed with 3 commercial varieties (Colegio 64, Negro and Sandía), from which 11 materials were obtained with characteristics accepted by consumers. To determine the genetic relationship of these materials with their parents, the PCR-RAPDs technique was used. Twenty oligonucleotides were tested and five of these exhibited polymorphism among the three commercial varieties. Later, these varieties were compared with 11 genotypes using the selected oligonucleotides. The genetic relationship of the 11 materials to the three commercial varieties was determined by the Jacard index. Results showed that 86% of the studied materials had a high degree of similarity, with genetic distances that varied from 0.825 to 1 for group one (two genotypes) and from 0.083 to 0.101 for group two (twelve genotypes).
Palavras-chave : Chilaca; RAPD-PCR; oligonucleotide; polymorphism; genetic distance.