Abstract

AUREOLES-RODRIGUEZ, F. et al. In vitro propagation of 'Maguey bruto' (Agave inaequidens Koch), an endangered species with economical interest. Rev. Chapingo Ser.Hortic [online]. 2008, vol.14, n.3, pp.263-269. ISSN 2007-4034.

'Maguey bruto' (Agave inaequidens Koch) is a wild, little studied endangered species. It is used to make the alcoholic spirit "Raicilla". To develop a method for reproduction to increase the population, several explants, concentrations of hormone, inorganic Murashige and Skoog (1962) salts, plant sizes and root conditions were evaluated in vitro. Some variables showed statistical significance. The stem sections formed 72 shoots in 8 weeks; the concentration of 3.0 mg·liter^-1 of 6-benziladenine (BA) produced an increase in length and number of shoots (6.39 mm and 2.02 respectively). The concentration of BA, kinetin (Kin) and 6-γ,γ-dimethylallylamino purine (2ip) produced a wide variety of responses: larger plants in a medium with MS salts at 100% concentration produced more and larger roots in less time, and 100% of the plants larger than 4.0 cm with more than 2 roots survived.

Keywords : raicilla; alcoholic spirit; cytokinins; tissue culture.

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