Resumo

TOVAR HERNANDEZ, Homero et al. Detection of mutations related to herbicide resistance in wild oat (Avena Fatua L.). Agric. Téc. Méx [online]. 2008, vol.34, n.2, pp.251-256. ISSN 0568-2517.

Two oligonucleotid groups were evaluated in order to detect two punctual mutations of the gene acetyl-CoA carboxilase (ACCasa) in Avena fatua L., which are related to the resistance to herbicides that inhibit fatty acid synthesis. Four populations of wild oat, two resistant (M5r and M28r) and two susceptible (M25s and M43s), to field application of clodinafop propargil were selected. Two groups of oligonucleotids were developed based on the sequence of the gene of the ACCasa from wild oat (GeneBank access number AF231334). The first group, VSE11GM, VRE11RGM, ACVII 11GM and ACVII 11RGM, was designed to detect the change of timine (T) by adenine (A) in position 1 322 of the described gene and which originated the change of isoleucine (ATT) by asparagine (AAT). The second group of oligonucleotids, VRDICGM, VRDITRGM, ACVRG1GM and ACVRG1RGM, was designed to detect the change of citosine or timine (T or C) by adenine (A) in position 541 of the same gene, and which originated the change of isoleucine (ATA) by leucine (CTA or TTA). Consequently, it was not possible to differentiate the populations that offered resistance in field from those which were susceptible and which would cause the change of isoleucine by leucine in the position 541 of the gene for both mutations. The absence of amplification of the fragment of 480 pb for the resistant population M5r, with the first group of oligonucleotids, as well as the absence of the amplification of the fragments 496 pb and 330 pb in the four populations with the second group of oligonucleotids, indicated the probability that it is a different substitution the one that confers resistance to the wild oat toward this group of herbicides.

Palavras-chave : Acetyl-CoA carboxilase; fatty acid synthesis; herbicides; mutation; resistance.

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