SciELO - Scientific Electronic Library Online

 
vol.42 número4Resistencia antibiótica de genotipos de cepas de Salmonella spp de cerdos sacrificados en rastros del Estado de MéxicoGenotoxicidad de la furazolidona y la forma libre de su metabolito 3-amino-2-oxazolidona, mediante la prueba de micronúcleos en linfocitos humanos índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Veterinaria México

versión impresa ISSN 0301-5092

Resumen

CARMONA-GASCA, Carlos Alfredo et al. Detection of Leptospira santarosai and L. kirschneri in cattle: new isolates with potential impact in bovine production and public health. Vet. Méx [online]. 2011, vol.42, n.4, pp. 277-288. ISSN 0301-5092.

Bovine leptospirosis causes high economic losses in cattle mainly due to reproductive failure, as well as representing public health risk. Since the last century, antibody titers against several Leptospira serovars have been detected by the microscopic agglutination test (MAT) in Mexico. With the exception of very few cases, the presence of serovars causing leptospirosis in cattle and other animal species has not been demonstrated by isolation in Mexico, and in such cases characterization had to be done abroad by complex and slow immunological approaches, by comparison with a number of reference strains. The present study was conducted to perform the molecular characterization of Leptospira isolates by multiple locus sequencing typing (MLST). A hundred and ninety seven sera and kidneys samples were collected immediately after slaughter, from grazing cattle coming from the south-eastern states of Mexico. Anti-Leptospira antibodies were detected by the MAT and kidneys were inoculated into EMJH and Fletcher's specific medium. A seropositivity of 60.4% (119 out of 197), with titers from 1:100 up to 1:3 200 was detected. Four isolates (2.03%), referred as CAL4, CAL6, CAL7 and MOCA45, were characterized by serology, ribotyping and MLST as L. kirschneri serovar Grippotyphosa; L. interrogans serovar Hardjo; L. santarosai serovar Mini and L. santarosai serovar Tarassovi, respectively. With the exception of serovar Hardjo, the three other isolates belong to serovars and species not previously isolated in Mexico. These findings make it necessary to evaluate the potential distribution of such serovars among cattle and their role on animal production and public health.

Palabras llave : Leptospira; Tarassovi; mini; grippotyphosa; cattle; isolation; MLST.

        · resumen en Español     · texto en Español     · pdf en Español