Abstract

OJEDA-MORALES, Marcia Eugenia et al. Three Proteus sp inoculant dose in crude oil biodegradation. Rev. Int. Contam. Ambient [online]. 2012, vol.28, n.4, pp.323-332. ISSN 0188-4999.

For several decades have existed in Mexico large areas impacted by oil spills. The majority of current bioremediation technologies use microorganisms to clean up oil sites. In this work, were prepared and evaluated three doses of Proteus sp. inoculants on clay-loam soil contaminated with hydrocarbons. The soil samples were collected in the municipality of Cunduacán, Tabasco, México. We determined the amount of total petroleum hydrocarbons (TPH) removed and the magnitude of population development of microorganisms during the study, using a factorial experimental design 3x3x3: three concentrations of HTP (treatments T_{1=50 000 ppm}, T_{2=70 000 ppm}, T_{3=90 000 ppm}), three times seed development of the microorganism pre-planted in the contaminated soil (D_{1=48 h} D₂=_{72 h} D₃=_{96 h}) and three replications. The data were analyzed using ANOVA procedure and mean test using the statistical software SAS-6. The dose that showed the highest degradation within 90 d was D1 in all treat-ments: 70.99 % (T1), 75.58 % (T2) and 67.56 % (T3) with late bacterial population 3.506x10³, 4.100x10³ and 3.867x10³ CFU/g of soil, respectively. Therefore, it is recommended to prepare the inoculum 48 h before being discharged into the clay-loam soil contamined with Istmo crude petroleum, and conditioning with Triple 17 in order to perform bioremediation.

Keywords : biodegradation of hydrocarbons; Proteus sp; inoculum dose; bioremediation.

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