Abstract

JIMENEZ ISLAS, Donaji et al. Fermentable sugar  production throug acid hydrolysis of Beta vulgaris L.. Rev. Int. Contam. Ambient [online]. 2012, vol.28, n.2, pp.151-158. ISSN 0188-4999.

With the purpose of increasing the amount of sugars that benefit the yield of ethanol in fermentation, it was performed hydrolysis of Beta vulgaris L. with H₂SO₄. The following parameters were used to evaluate the degree of hydrolysis of substrate: stirring, acid concentration, mass, and temperature. The mass was tested in the range of 5 to 40 g and stirring from 100 to 250 rpm. The temperature and reaction time were evaluated in the range of 30 to 90 °C and 2 to 6 h, respectively. The tested particle size was 0.5 mm and a reaction volume of 50 mL was used for all cases. The rate of 150 rpm had a better effect over hydrolysis. By comparison of means it was determined that concentrations higher than 0.5 N of H₂SO₄ showed no significant effect (p<0.05) on the release of total sugars. The highest yield of hydrolysis was obtained with 10 g of substrate in the reaction medium. The temperature of 90 °C provided the highest hydrolysis and at 50 °C the amount of total sugars was 3 g/L lower than that generated with 90 °C. The best pretreatment conditions were: 40 °C, 150 rpm and 10 g of substrate. The increase in the reaction time indicates that hemicellulose degradation occurs inside the matrix of substrate and that amorphous regions are generated, promoting cellulose availability.

Keywords : simple sugars; red beet (Beta vulgaris L); bioethanol; pretreatment.

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