Resumo

SALMONES, Dulce  e  MATA, Gerardo. Laccase production by Pleurotus djamor in agar media and during cultivation on wheat straw. Rev. Mex. Mic [online]. 2015, vol.42, pp.17-23. ISSN 0187-3180.

Laccase production in vitro and mycelial growth were measured for seventy one Pleurotus djamor strains cultivated on malt extract agar (MEA) or yeast malt extract agar (YMEA) supplemented with water soluble lignin derivatives (WSLD). In vitro laccase production and mycelial diameters averages were higher in the YMEA-WSLD cultures than MEA cultures. The four strains with the highest enzyme production in vitro were selected to measure their laccase activity pattern during the culture cycle, using wheat straw as substrate. Laccase activity was detected on second day of incubation. The first peak of activity (169.8-579.8 μMol g^-1 min^-1) was reached on day 4, and the second peak of activity was observed during the initiation and development of fruiting bodies, with values from 164.4 to 469.9 μMol g^-1 min^-1 measured. These results suggest that a laccase system in P. djamor is associated with the reproductive stage. Furthermore, high laccase production observed in these strains evidences that P. djamor may be an interesting species for use in some biotechnological processes.

Palavras-chave : mushroom cultivation; enzymes; biomass; mycelial growth.

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