Abstract

ROMAN, Rosa; TORRES-DUARTE, Cristina; AYALA, Marcela  and  VAZQUEZ-DUHALT, Rafael. Production of laccase from Coriolopsis gallica at pilot plant scale. Rev. Mex. Mic [online]. 2010, vol.32, pp.19-27. ISSN 0187-3180.

The production of laccase from the ligninolytic fungi Coriolopsis gallica was carried out at pilot plant scale in a 100 L bioreactor. A cheap fermentation medium was used containing 3% Bran Flakes in 60 mM phosphate buffer, pH 6.0 containing 0.8 mM copper sulfate. A total of 630 650 laccase units were obtained in 7-days cultures. After few purification steps, including centrifugation, ultracentrifugation, ammonium sulfate precipitation, and two ionic exchange chromatographies, a partially purified preparation was obtained with 79% of laccase showing a specific activity of 313 U/mg.

Keywords : fungal laccases; enzyme production; white rot fungi.

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