Abstract

IGLESIAS-ANDREU, L. et al. RAPD markers associated with sex expression in Ceratozamia mexicana Brongniart (Zamiaceae). Rev. Chapingo [online]. 2010, vol.16, n.2, pp. 139-145. ISSN 0186-3231.  http://dx.doi.org/10.5154/r.rchscfa.2010.04.016.

Ceratozamia mexicana Brongniart is a strictly dioecious species with a long juvenile stage. In order to detect molecular markers associated with sex, it was analyzed by the technique of random amplified polymorphic DNA (RAPD) and masal segregant analysis (BSA) samples of leaf tissue of leaf tissue of sexually differentiated individuals of the population of this species located at Coacoatzintla, Veracruz, México. 5 primers were used to amplify three bulk DNA samples of five known individuals from each sex. A total of 37 bands were produced of which 63% were polymorphic. The OPB08 and OPK10 primers generated distinctive bands to male sex in C. mexicana. With the OPK10 primer a band of 500 bp was observed in two of the three male bulk samples tested, while the OPB08 primer detected two bands (1400 and 1100 bp) in the male samples tested. Although it is necessary to validate this observation, these results could open a new alternative to early determination of sex in C. mexicana.

Keywords : Ceratozamia mexicana; sex identification; RAPD; molecular markers; bulked segregate analysis (BSA).