Abstract

LEE, C-W et al. Isolation and characterization of culturable bacteria from tropical coastal waters. Cienc. mar [online]. 2009, vol.35, n.2, pp.153-167. ISSN 0185-3880.

In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 x 10³ cfu mL^-1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (Ddel, Hhal, Rsal, and Sau3AI), of which Hhal gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of γ-Proteobacteria (61.1%); 3 strains of α-Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-B-hydroxybutyrate.

Keywords : 16S rDNA RFLP; marine bacteria; South China Sea; Straits of Malacca; ZoBell 2216E.

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