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Revista mexicana de fitopatología
versión On-line ISSN 2007-8080versión impresa ISSN 0185-3309
Resumen
OCHOA-MARTINEZ, Daniel Leobardo et al. Cymbidium mosaic virus and Odontoglossum ringspot virus in various genera of cultivated orchids in Mexico. Rev. mex. fitopatol [online]. 2019, vol.37, n.3, pp.421-431. Epub 30-Sep-2020. ISSN 2007-8080. https://doi.org/10.18781/r.mex.fit.1903-1.
In the Miguel Ángel Soto Arenas Orchid, of the UNAM Faculty of Sciences, plants with symptoms consisting of chlorosis, variegated, necrosis and mosaic have been detected. Several of these plants are endemic species of Mexico, and there is no information on their phytosanitary management. Therefore, the objective of the present study was to know the causal agent or agents of these symptoms through ELISA tests, RT-PCR, differential plants and phylogenetic analyzes. Odontoglossum ringspot virus (ORSV) and Cymbidium mosaic virus (CymMV) were detected in 89% of the sampled plants with symptoms. Differential plants (Nicotiana tabacum var. xhanti, N. glutinosa, Chenopodium quinoa, C. amaranthicolor and Datura stramonium) shown local lessions after six days of the mechanical inoculation in both viruses; And only using CymMV, N. benthamiana was systemically infected. The phylogenetic analyses showed that these viruses were grouped with isolates from Asian countries suggesting that the infection probably originated from the exchange of plant material between producers. This is the first report of CymMV and ORSV infecting Barkeria, Lycaste, Rossioglossum, Masdevallia, Leochilus, Stanhopea, Maxillaria, Epidendrum sec. Oerstedella, Dendrobium, Sobralia and Cuitlauzina in Mexico.
Palabras llave : Barkeria; Lycaste; Dendrobium; tobamovirus; potexvirus..