Resumen

VALADEZ-RAMIREZ, Pedro et al. New records of Cucumber mosaic virus isolates and associated-satellite RNA in Colima, Mexico. Rev. mex. fitopatol [online]. 2019, vol.37, n.2, pp.357-364.  Epub 30-Sep-2020. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1901-2.

Cucumber mosaic virus (CMV) causes one of the most important viral diseases in wild and cultivated plants worldwide. Research on CMV in Mexico has been scarce, and due to the economic impact on plant health and wide host range, it is necessary to carry out studies on its presence and distribution in the agricultural geographical areas of the state of Colima. In this study, four CMV isolates identified by RT-PCR, DNA sequencing and phylogenetic analysis are reported: CMV-Vin from periwinkle (Catharanthus roseus), CMV-Chi from jalapeño pepper (Capsicum annuum), and CMV-Tom from saladette tomato (Solanum lycopersicum). In addition, the presence of CMV from cantaloupe melon (Cucumis melo) (CMV-Mel) was confirmed. The nucleotide sequences from CMV-Vin, CMV-Chi, and CMV-Mel clustered with CMV isolates from subgroup IB, while CMV-Tom clustered with members of subgroup IA. A satellite RNA sequence, without necrogenic domain, was detected only in CMV-Vin (satRNA Vin). This study reports for the first time the presence of CMV in periwinkle, pepper and tomato plants, and of an RNA satellite from periwinkle in Colima, Mexico.

Palabras llave : CMV isolates; CARNA-5; RT-PCR; DNA sequencing; phylogenetic analyses.

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