Abstract

GUTIERREZ-JIMENEZ, Eduardo et al. Effect of natural oils against Mycosphaerella fijiensis under in vitro conditions and detection of active plant chemicals. Rev. mex. fitopatol [online]. 2018, vol.36, n.1, pp.141-150. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1707-4.

Chemical control of black sigatoka in banana Mycosphaerella fijiensis has increased the selection pressure on the pathogen, a fact which in turn creates an environmental impact. The objective of this study was to evaluate the effect of essential Pimenta dioica, Piper auritum, Sysygium aromaticum, Cinnamomum zeylanicum, Origanum vulgare, Artemisia ludoviciana, and Origanum majorana oils on M. fijiensis growth under in vitro conditions, and to identify their active metabolites. The oils were obtained by hydrodistillation. M. fijiensis was isolated and developed on PDA culture medium. Thin layer chromatography (TLC) and column chromatography (CC) were used to identify the metabolites of each essential oil. Five concentrations: 50, 100, 500, 1000 y 5000 ppm of each oil were used. P. dioica, C. zeylanicum and O. vulgare oils had a significant effect with lower M. fijiensis mycelial growth at a concentration of 500 ppm, whereas the effect on O. majorana and A. ludoviciana was observed at a concentration of 1000 ppm. Cinnamic aldehyde was the main metabolite detected in C. zeylanicum species, and eugenol and carvacrol in P. dioica y O. vulgare species.

Keywords : plants extract; secondary metabolites; fungi inhibition; biological control.

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