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Revista mexicana de fitopatología

On-line version ISSN 2007-8080Print version ISSN 0185-3309

Abstract

BERMUDEZ-GUZMAN, Manuel de Jesús et al. Presence of Papaya ringspot virus (PRSV) in weed associated with Carica papaya in Colima, Mexico. Rev. mex. fitopatol [online]. 2018, vol.36, n.1, pp.1-15. ISSN 2007-8080.  http://dx.doi.org/10.18781/r.mex.fit.1706-2.

Papaya ringspot virus (PRSV) is a Potyvirus of economic importance for papaya crops (Carica papaya L.) whose main symptoms are mosaic, chlorosis and deformation in leaves, as well as ring-like characteristic spots on the fruit. In Mexico, there is no report on weed species associated with this crop that act as reservoir of Potyvirus. In the present study, the presence of PRSV in weeds of commercial papaya plantations was identified in the state of Colima, Mexico. In a sample of 139 papaya plants and 70 weed samples with symptoms of viral infections, PRSV was detected in 40 and 50% for Carica papaya and Cucurbita pepo, respectively, using DAS-ELISA (Double antibody sandwich enzyme-linked immunosorbent assays). Nucleotide sequence analyses of the 350 base pairs fragments of the PRbV NIb region generated by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) allowed the identification of Potyvirus in 40-90% of the total samples of Abutilon abutilastrum and Anoda cristata (Malvaceae), as well as Solanum rostratum (Solanaceaea), which represents a new report on PRSV reservoir weeds associated with papaya crops in Mexico.

Keywords : weed; alternate hosts; DAS-ELISA; RT-PCR; Potyvirus.

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