SciELO - Scientific Electronic Library Online

 
vol.35 número3Reducción en la incidencia de la pudrición gris en uva de mesa por el efecto de volátiles de un extracto de ajoResistencia genética a Sporisorium reilianum f. sp. zeae en líneas seleccionadas de maíz (Zea mays L.) con endospermo blanco y amarillo índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Revista mexicana de fitopatología

versión On-line ISSN 2007-8080versión impresa ISSN 0185-3309

Resumen

OBRADOR-SANCHEZ, José Abraham; TZEC-SIMA, Miguel; CANTO-CANCHE, Blondy  y  HIGUERA-CIAPARA, Inocencio. Techniques for isolation, identification and molecular characterization of Moko disease-related Ralstonia solanacearum strains. Rev. mex. fitopatol [online]. 2017, vol.35, n.3, pp.509-533. ISSN 2007-8080.  http://dx.doi.org/10.18781/r.mex.fit.1705-1.

Ralstonia solanacearum is one of the most destructive phytopathogens in the agriculture. In banana cultivation, the race 2 causes Moko disease, which is present in some areas in Mexico, and when epidemic outbreaks burst there are losses. Race 2 comprises subgroups (sequevars) which are usually identified by Multiplex PCR reactions. Isolation of R. solanacearum is usually hard, even on semiselective media, because it grows slowly and it is frequently contaminated with Pseudomonas, Klebsiella and Erwinia bacteria, which show similar phenotypes. This work shows a strategy that make easy the isolation, identification and classification of R. solanacearum race 2. The protocol comprises immunodiagnosis of plant suspicious samples, confirmation of diagnosis by PCR, isolation on semiselective media SMSA and B-King, and genotyping of sequevars by single, specific PCR reactions. In the present work 25 strains were isolated from Tabasco, and they genotyped in sequevar 6. Their previous genotyping by Multiplex yielded confuse results, which make evident that Multiplex can result in incorrect conclusions. We discourage to propose genetic divergence and emergency of new strains based on Multiplex results, as done in some recent reports.

Palabras llave : Banana; diagnosis; bacterial isolation; genotyping; sequevar.

        · resumen en Español     · texto en Español | Inglés     · Español ( pdf ) | Inglés ( pdf )