SciELO - Scientific Electronic Library Online

 
vol.35 número1Las cenicillas en cultivos agrícolas de Sinaloa: Situación actual sobre su identificación y líneas futuras de investigaciónIn vitro primer reporte del efecto de nutrimentos y del silicio en el crecimiento de Phaeocryptopus gaeumannii, un patógeno de Pseudotsuga menziesii índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Revista mexicana de fitopatología

versión On-line ISSN 2007-8080versión impresa ISSN 0185-3309

Resumen

VARGAS-ARISPURO, Irasema et al. Looking for natural antifungal agent: Sensitive assey to detect fungal endo-1,3-β-glucanase inhibitors from crude plant extracts. Rev. mex. fitopatol [online]. 2017, vol.35, n.1, pp.130-138. ISSN 2007-8080.  http://dx.doi.org/10.18781/r.mex.fit.1606-5.

1,3-β-glucanase is an enzyme involved in fungal cell wall construction, division septum deposition and ascospore wall assembly. Hence, the 1,3-β-glucanase is a target site to develop into new generations of natural antifungal agents. With the aim of seeking antifungal compounds in plant extracts, we implemented a simple and sensitive assay to detect inhibitors of endo-1,3-β-glucanase from crude plant extracts. The assay, especially useful for screen large number of crude plant extracts, is based on the diffusion of glucanase enzyme from wells through agarosa gel containing the enzyme substrate (laminarin). As the substrate is depolymerized, glucanase diffuses outward in a radial manner leaving a circular zone around the well, unstained by dye calcofluor. A linear relationship between the diameter of hydrolyzed zone and pure enzyme concentration, provide the basis for the method. Results of the assay using plant extracts obtained in methanol or dichloromethane are discussing.

Palabras llave : Natural fungicide; fungal target site; plant extracts; hydrolase.

        · resumen en Español     · texto en Español | Inglés     · Español ( pdf ) | Inglés ( pdf )