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Revista mexicana de fitopatología

On-line version ISSN 2007-8080Print version ISSN 0185-3309

Abstract

RAMIREZ-ROJAS, Sergio et al. Detection of Iris yellow spot virus in onion plants from Tepalcingo, Morelos state, Mexico. Rev. mex. fitopatol [online]. 2016, vol.34, n.3, pp.308-315. ISSN 2007-8080.  http://dx.doi.org/10.18781/r.mex.fit.1604-1.

In Morelos state, recently have been observed viral diseases in onion (Allium cepa); one of them is caused by the Iris yellow spot virus (IYSV) which belong to the Bunyaviridae family and the Tospovirus gender and is transmitted by Thrips tabaci Lindeman (Thysanoptera: Thripidae) to onion plants. In 2012, there was a 100 % incidence of IYSV and severity of over 90 % on 2,500 ha of commercial crop. The objective of this research was to identify the presence of IYSV through real time RT-PCR and sequencing of the virus. To accomplish this, leaves were sampled from commercial fields at Tepalcingo, Morelos, from transplanting to harvest. Total RNA extraction was done with TRIzol® Reagent. Virus detection was done using specific primers for the nucleoprotein gen of IYSV giving as a result the amplification of a specific product through real-time RT-PCR, and an expected strip of 896 bp, which after sequencing confirmed 99 % identity with the nucleoprotein gen of the virus. The IYSV virus was detected in onion plants from Tepalcingo, Morelos and the sequence obtained was registered in the database of the GenBank (JX946658).

Keywords : Real time RT-PCR; Tospovirus; Thrips tabaci; Onion yellow spot.

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