Abstract

ROBLEDO-LEAL, Efrén et al. Isolation of killer yeasts from ants of the genus Atta and their effect on the red tomato’s fungal pathogen Geotrichum candidum. Rev. mex. fitopatol [online]. 2016, vol.34, n.3, pp.258-269. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1605-3.

One of the diseases occurring in tomato is the sour rot caused by Geotrichum candidum. In order to evaluate the antagonism of killer yeasts, isolates were obtained from ants belonging to the genus Atta and those with killer activity were selected, resulting in 8 killer isolates (M1 - M8). Then, isolates of G. candidum were obtained from infected tomatoes and the most aggressive one was selected. Each killer yeast was inoculated in 1x1 cm2 cuts made to tomato fruits and after a 3 hour incubation, 50 μL of a G. candidum suspension of 1x104 cells/mL was inoculated. After incubation for 72 h in a moist chamber, the lesions were quantified using the Tukey test (p<0.05) resulting in yeast isolates M1 and M2 as the most protective ones. The auxanogram test API 20C AUX identified them as Candida (Pichia) guilliermondii.

Keywords : Killer yeasts; antagonism; Pichia guilliermondii; Geotrichum candidum.

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