Abstract

LUGO MELCHOR, Ofelia Yadira; GUZMAN URIARTE, Roberto; GARCIA ESTRADA, Raymundo Saúl  and  LEON FELIX, Josefina. Geminivirus Transmitted by White Fly (Bemisia tabaci) in Tomato, in the Agricultural Valley of Culiacan, Sinaloa. Rev. mex. fitopatol [online]. 2011, vol.29, n.2, pp.109-118. ISSN 2007-8080.

Sinaloa is one of the main tomato producer states in Mexico; however, its tomato production has been seriously affected by diseases related to the presence of geminivirus transmitted by whitefly, causing great economic losses for the state. in this study, geminivirus present in tomato plants were identified and characterized by nested polymerase chain reaction (PCR) and later sequencing analysis. A total of 507 plants were analyzed, from which, 197 plants showed symptoms of the presence of geminivirus and 310 plants did not show any symptoms of the presence of geminivirus. After analysis, 134 samples were found positive, 99 (73.8%) corresponding to plants that presented symptoms, and 35 (26.1%) without symptoms. From the 134 positives samples detected by nested PCR, 64.2% belonged to TYLCV, 22.4% to SLCV, 6.7% were variants of other viruses (STLCV AV1, PHYVV, CuLCrV segment A and B) and 3.7% contained geminivirus whose type was not identified. The nested PCR allowed detection of geminivirus transmitted by whitefly in tomato plants with, and without symptoms. Posterior sequencing analysis allowed to determine the different types of virus, as well as, new variants of geminivirus which has not been previously detected in tomato plants.

Keywords : Solanum lycopersicum; molecular.

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