Abstract

RODRIGUEZ PAGAZA, Yolanda et al. Optimal Conditions to Induce Eruptive and No Eruptive Germination of Sclerotium cepivorum and Sequences Associated to Protein Synthesis during No Eruptive Germination. Rev. mex. fitopatol [online]. 2011, vol.29, n.1, pp.69-72. ISSN 2007-8080.

A subtractive library of sequences associated to proteins synthesis in hyphal germination of Sclerotium cepivorum was generated. In order to generate the subtractive library, optimal conditions to have eruptive and hyphal germination of S. cepivorum sclerotia were determined. At 22° C, 96.7% of sclerotia germinated hyphally without allyl disulfide (DAS) and 91.67% of sclerotia germinated eruptively in presence of DAS 2%. Twenty three fragments of genes associated to hyphal germination of S. cepivorum were obtained in the subtractive library. Those fragments had significant similarity to 15 different proteins that participate in respiration, transcription, translation, signal transduction and amino acid and protein synthesis process. The results suggest that hyphal germination is faster than eruptive germination because have an increment in the metabolic processes in sclerotia of S. cepivorum germinated hyphally.

Keywords : Substractive hybridization; allyl disulfide; GenBank.

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