Abstract

HERNANDEZ-DE LA CRUZ, Miguel et al. Serological and molecular detection of virus PVY^N and its variant PVY^NTN in potato (Solanum tuberosum L.) and alternate hosts in Tapalpa, Mexico. Rev. mex. fitopatol [online]. 2007, vol.25, n.2, pp.167-172. ISSN 2007-8080.

Sampling for the presence of PVY^N virus and its variant PVY^NTN, as well as for weeds and insects associated with cultivation of potato (Solanum tuberosum), was carried out in fields intended for seed production in Tapalpa, State of Jalisco, Mexico. Forty one cultivars were analyzed, 19 from Tapalpa and 22 from Toluca, State of Mexico, by serological and molecular techniques. DAS-ELISA immunoassays revealed that cv. Malinche from Tapalpa was positive to PVY^N, whereas a RT-PCR-based analysis identified the PVY^NTN variant in Marinca from Toluca, as well as in Gigant, Malinche, and Alpha from Tapalpa. Among the different weed species associated to potato crop, Solanum nigrescens locally known as hierba mora, was found to be a natural reservoir for PVY^N. The aphid Myzus persicae was identified to carry PVY^NTN variant and it is considered as a probable vector. A 594 bp fragment, derived from samples that resulted positive for PVY^NTN was amplified by RT-PCR, cloned and sequenced with 98% homology to an isolate from Canada, according to the sequence reported in the NCBI. These results confirmed the presence of the PVY^NTN variant in commercial potato fields in Tapalpa, Jalisco, Mexico.

Keywords : Myzus persicae; Solanum nigrescens; Solanum nigrum; DAS-ELISA; RT-PCR.

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