SciELO - Scientific Electronic Library Online

vol.33 número1Demografía de brúquidos (Coleoptera: Bruchidae) asociados con Vigna unguiculata (L.) Walp (Fabaceae)Presencia espacial y temporal de aves rapaces diurnas (Aves: Accipitriformes, Falconiformes) en Marismas Nacionales, Nayarit-Sinaloa, México índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados

  • No hay artículos similaresSimilares en SciELO


Acta zoológica mexicana

versión On-line ISSN 2448-8445versión impresa ISSN 0065-1737


SOSA-CASTILLO, María Elena; LARA REYNA, Joel; ORTEGA ARENAS, Laura Delia  y  HERNANDEZ, Alfonsina Judith. Standardization and validation of the nested PCR test for the diagnosis of the genus Xyleborus species (Coleoptera: Curculionidae: scolytinae). Acta Zool. Mex [online]. 2017, vol.33, n.1, pp.18-26. ISSN 2448-8445.

In this study it was standardized and validated the nested PCR test for rapid, sensitive and reliable detection of species of the genus Xyleborus using external “primers” CI-J-2183 and TL2-N-3014 and internal primers J2210 and N2739 that amplify a band of 500 bp in the region of the gene mitochondrial Cytochrome Oxidase subunit 1(CO1). Also, DNA extraction from 26 specimens of Xyleborus was realized with kit Qiagen DNeasy ® mericom Food (DMF), not previously reported its use for their application in insects, which resulted in enough DNA and high-quality for amplification by PCR reactions. The method allowed to process a single insect by extraction, and obtain genetic material from specimens preserved in alcohol of up to eight years old. The detection limit was defined up to a concentration of 780 pg/μL. The PCR was optimized in a final volume of 15 µL without compromising quality of amplification. The standardized test allowed quality DNA, which ensured high reproducibility and sensitivity in the detection of species of Xyleborus and partial sequencing of the CO1 gene to the seven species studied; consensus sequences were analyzed by homology and deposited in the GenBank.

Palabras llave : Ambrosial beetles; molecular identification; diagnosis; CO1 gene.

        · resumen en Español     · texto en Español     · Español ( pdf )