SciELO - Scientific Electronic Library Online

 
vol.31 número1Diversidad genética de dos especies de abejas sin aguijón, Trigona nigerrima (Cresson) y Trigona corvina (Cockerell) en paisajes cafetaleros del Sureste de MéxicoEspecies de Thysanoptera en una localidad del Cerro de la Muerte en Costa Rica, Centro América índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Acta zoológica mexicana

versão On-line ISSN 2448-8445versão impressa ISSN 0065-1737

Resumo

CASTILLO-MARTINEZ, Antonio et al. Detection of Rickettsia sp. in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) of Matamoros, Coahuila, Mexico. Acta Zool. Mex [online]. 2015, vol.31, n.1, pp.80-83. ISSN 2448-8445.

Two hundred and seventeen ticks randomly collected from seventy two domestic dogs, in Matamoros municipality, Coahuila, these were taxonomically identified as Rhipicephalus sanguineus Latreille. In order to determine Rickettsia sp. presence in ticks these were grouped in 100 pools for molecular testing. Engorged female ticks were dissected to obtain pellets containing internal organs and haemolymph. Nymphs and males were triturated for exposing internal contents from which DNA was extracted by the CTAB technique and subjected to PCR to amplify the gltA gene for detection of Rickettsia. The frequency for Rickettsia sp. was 4% (4). The present work constitutes the first record of the causative agent of the spotted fever Rickettsia sp. in internal organs of brown dog tick R. sanguineus through molecular techniques in Matamoros municipality, Coahuila, endemic region of this disease.

Palavras-chave : Rickettsia spp.; PCR; ticks; gltA.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons