Resumen

CASTILLO-MARTINEZ, Antonio et al. Detection of Rickettsia sp. in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) of Matamoros, Coahuila, Mexico. Acta Zool. Mex [online]. 2015, vol.31, n.1, pp.80-83. ISSN 2448-8445.

Two hundred and seventeen ticks randomly collected from seventy two domestic dogs, in Matamoros municipality, Coahuila, these were taxonomically identified as Rhipicephalus sanguineus Latreille. In order to determine Rickettsia sp. presence in ticks these were grouped in 100 pools for molecular testing. Engorged female ticks were dissected to obtain pellets containing internal organs and haemolymph. Nymphs and males were triturated for exposing internal contents from which DNA was extracted by the CTAB technique and subjected to PCR to amplify the gltA gene for detection of Rickettsia. The frequency for Rickettsia sp. was 4% (4). The present work constitutes the first record of the causative agent of the spotted fever Rickettsia sp. in internal organs of brown dog tick R. sanguineus through molecular techniques in Matamoros municipality, Coahuila, endemic region of this disease.

Palabras llave : Rickettsia spp.; PCR; ticks; gltA.

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