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Salud Pública de México
versión impresa ISSN 0036-3634
Resumen
GLEASON-RODRIGUEZ, Graciela et al. Dengue virus infection induces chromatin remodeling at locus AAEL006536 in the midgut of Aedes aegypti. Salud pública Méx [online]. 2018, vol.60, n.1, pp.41-47. ISSN 0036-3634. https://doi.org/10.21149/8471.
Objective:
To identify and characterize Aedes aegypti’s AAEL006536 gene proximal upstream cis-regulatory sequences activated by dengue virus infection.
Materials and methods:
A. aegypti Rockefeller strain mosquitoes were blood fed or infected with dengue virus 2. Open chromatin profiling was then carried out in pools of midguts from each group of mosquitoes.
Results:
The proximal upstream region does not contain open chromatin sites in the midguts of blood-fed mosquitoes as detected by FAIRE-qPCR. In contrast, two cis-regulatory sites were identified in the same upstream region of dengue virus-infected mosquito midguts. The distal sequence contains STAT-, REL- and C/EBP-type transcription factor binding sites.
Conclusion:
The activation of two proximal cis-regulatory sequences, induced by dengue virus infection, is mediated by chromatin remodeling mechanisms. Binding sites suggest a dengue virus infection-induced participation of immunity transcription factors in the up-regulation of this gene. This suggests the participation of the AAEL006536 gene in the mosquito’s antiviral innate immune response.
Palabras llave : Dengue virus; A. aegypti; insect vector; transcriptional activation; chromatin; viral infection; transcription factor; binding site; innate immunity.
