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Revista mexicana de física

versión impresa ISSN 0035-001X

Resumen

RAMIREZ MIQUET, E. E.; MARTI LOPEZ, L.  y  CONTRERAS ALARCON, O.R.. Dos configuraciones diferentes para la descripción temporal de actividad de Escherichia coli mediante speckle dinámico. Rev. mex. fis. [online]. 2011, vol.57, n.5, pp.441-445. ISSN 0035-001X.

We present the experimental results of applying the laser dynamic speckle technique to describe bacterial cells activity corresponding to the species Escherichia coli (ATCC 25922) cultivated in two-compartment Petri dishes, using one of them as a non-inoculated control blank. In the experimental series we registered speckled images and after processing them, we obtained optical signals of the bacterial activity corresponding to a single cell concentration and two different laser beam impact configurations. The results for both configurations are discussed. We conclude that the method, the designed and mounted set-up and the associated image processing allowed the detection and description of the bacterial activity.

Palabras llave : Dynamic speckle; bacterial activity; inoculum; CFU; image processing.

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