Gaceta médica de México
versión impresa ISSN 0016-3813
CASTILLO, Carlos; CASTILLO, Enrique F.; LOPEZ, Jorge y LOPEZ, Ruth M.. Testosterone inhibits the contractile responses to phenilephrine associated with the release of intracellular calcium in rat aorta. Gac. Méd. Méx [online]. 2006, vol.142, n.1, pp. 1-8. ISSN 0016-3813.
Using endothelium-denuded rat aortic rings incubated in Ca2+-free solution, we assessed the ability of testosterone to influence the contractile effect of phenylephrine, and the increase in resting tone (IRT) associated with Ca2+ ability to cross the plasma membrane. The addition of testosterone[10-5-10-4M]5 min before phenylephrine [10-6 M], inhibited both phenylephrine-induced contraction and IRT. These changes were not affected by cycloheximide (10-5 M; a protein synthesis inhibitor of), flutamide (10-5 M; an androgenic receptor antagonist), or by adding aminoglutethimide (10-5 M; an aromatase inhibitor). Testosterone also blocked the contractile response to serotonin [10-5 M] but not to caffeine [10-2 M]. On the other hand, testosterone inhibited the contractile responses to cyclopiazonic acid (10-6 M; a selective Ca2+ -ATPase inhibitor) or ryanodine (10-5 M; an activator of sarcoplasmic reticulum Ca2+ -release channels) associated with capacitative Ca2+ influx through non-L-type Ca2+ channels. These data suggest that by acting on the cellular membrane, testosterone interferes with the signal transduction pathway of Gq-11 protein-coupled receptors, and inhibits capacitative Ca2+ influx through both L-type and non-L-type Ca2+ channels. These effects are non-genomic, non-mediated by the intracellular androgen receptor, and not due to the conversion of testosterone to estrogens.
Palabras llave : Testosterone; intracellular Ca2+ release; adrenergic agonists.