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Revista odontológica mexicana
versión impresa ISSN 1870-199X
Rev. Odont. Mex vol.11 no.1 Ciudad de México mar. 2007
https://doi.org/10.22201/fo.1870199xp.2007.11.1.15884
Trabajos originales
Estudio comparativo in vitro de la capacidad antibacteriana de la clorhexidina, hidróxido de calcio y yoduro de potasio yodado contra Fusobacterium nucleatum
Comparative in vitro study of Chlorhexidine, calcium hydroxide and iodine potassium iodide against Fusobacterium nucleatum
* Alumna de la Especialidad de Endodoncia. División de Estudios de Posgrado e Investigación de la Facultad de Odontología de la UNAM. México.
§ Jefe del Laboratorio de Genética Molecular. División de Estudios de Posgrado e Investigación de la Facultad de Odontología de la UNAM. México.
II Coordinador de la Especialidad de Endodoncia. División de Estudios de Posgrado e Investigación de la Facultad de Odontología de la UNAM. México.
Introducción
El tratamiento endodóncico tiene como uno de sus principales objetivos la eliminación de la microflora del sistema de conductos radiculares. Esto se logra mediante la limpieza, desinfección y conformación de dicho sistema mediante la instrumentación, irrigación y utilización de medicación intraconducto.
Objetivo
Comparar la capacidad para inhibir el crecimiento de Fusobacterium nucleatum con la utilización de tres medicamentos intraconducto: clorhexidina (CHX), hidróxido de calcio (Ca(OH)2) y yoduro de potasio yodado (IKI) utilizados individualmente y en diferentes combinaciones.
Metodología
F. nucleatum subespecie nucleatum fue sembrado en placas de agar. Discos de papel filtro fueron embebidos en cada medicamento y colocados sobre la superficie del agar. Las placas fueron incubadas en una cámara de anaerobiosis durante 7 días. Se efectuó el registro en milímetros de los radios de las zonas de inhibición del crecimiento.
Resultados
La capacidad antimicrobiana fue mayor para CHX, seguida por IKI y Ca(OH)2. La CHX al 2% inhibió significativamente mejor el crecimiento bacteriano que el IKI al 4% + 2% (p < 0.05). La CHX al 2% mostró una capacidad antimicrobiana significativamente mayor que cualquiera de las concentraciones de Ca(OH)2. Ninguna de las combinaciones de medicamentos intraconducto, mostraron capacidad antimicrobiana superior a la de CHX al 4% y 2% individualmente.
Conclusiones
La CHX al 4%, 2% y 1% de manera individual, presentó una actividad antimicrobiana elevada, seguida por el IKI con una actividad moderada y el Ca(OH)2 que presentó una escasa capacidad para inhibir el crecimiento de F. nucleatum. La combinación de medicamentos intraconducto no potenció los efectos antimicrobianos de los medicamentos evaluados.
Palabras clave: Medicamentos intraconducto; Fusobacterium nucleatum; clorhexidina; hidróxido de calcio; yoduro de potasio yodado
Introduction
One of the primary objectives of root canal therapy is the removal of microbial species within the canal system. Such goal is accomplished by cleansing, disinfecting and shaping of the canals through mechanical instrumentation, irrigation and the use of intracanal medicaments.
Purpose
To compare the effectiveness in inhibiting the growth of Fusobacterium nucleatum of 3 intracanal medicaments: chlorhexidine (CHX), calcium hydroxide (Ca(OH)2) and iodine potassium iodide (IKI) used individually and in various combinations.
Methods
F. nucleatum subspecies nucleatum was grown on agar plates. Paper filter disks were immersed in each medicament and placed on the agar surface. Plates were incubated in an anaerobic chamber for 7 days. The radius of growth-inhibition zones was recorded in millimeters.
Results
Antimicrobial effect was greater for CHX, followed by IKI and Ca(OH)2. 2% CHX inhibited significantly better the bacterial growth than 4% + 2% IKI (p < 0.05). Two percent CHX also exhibited a significantly greater antimicrobial effect than either of the concentrations of Ca(OH)2 tested. None of the combinations of medicaments exhibited a greater antimicrobial effect than 4% and 2% CHX tested individually.
Conclusions
4%, 2% and 1% CHX individually, exhibited the greatest antimicrobial effect followed by IKI with a moderate effect and Ca(OH)2 which exhibited only a discrete antimicrobial activity against F. nucleatum. The combination of intracanal medicaments did not increase the antimicrobial effects of the medicaments evaluated individually.
Keywords: Intracanal medicaments; Fusobacterium nucleatum; chlorhexidine; calcium hidroxide; iodine potassium iodide
Referencias
1. Walton RE, Torabinejad M. Endodoncia. Principios y práctica. Segunda Edición. McGraw-Hill. México. 1996: 298-301. [ Links ]
2. Cohen S, Burns RC. Vías de la pulpa. Octava Edición. Elsevier Science. Madrid. 2002: 493-497. [ Links ]
3. Ingle JI, Bakland LK. Endodoncia. Quinta Edición. McGraw-Hill Interamericana. México. 2004: 4-67. [ Links ]
4. Morrier JJ, Benay G, Hartmann C, Barsotti O. Antimicrobial activity of Ca(OH)2 dental cements: an in vitro study. J Endodon 2002; 29(1): 51-54. [ Links ]
5. Langeland K, Guldener PH. Endodoncia. Springer. México. 1995: 79-92. [ Links ]
6. Podbielski A, Spahr A, Haller B. Additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens. J Endodon 2003; 29(5): 340-345. [ Links ]
7. Slots J. Contemporary oral microbiology and immunology. McGraw-Hill. Boston, USA. 1992; 314-317. [ Links ]
8. Gomes BP, Sato E, Ferraz RC, Teixeira FB, Zaia AA, Souza-Filho FJ. Evaluation of time required for recommendation of coronally sealed canals medicated with calcium hydroxide and chlorhexidine. Int Endod J 2003; 36: 604-609. [ Links ]
9. Randi FC, Figuereido de Almeida GP, Zaia AA, Teixeira FB, Souza-Filho FJ. In vitro assessment of the antimicrobial action and the mechanical ability of chlorhexidine gel as an endodontic irrigant. J Endodon 2001; 27(7): 452-455. [ Links ]
10. Leonardo MR, Filho MT, Silva LA, Filho NP, Bonifacio KC, Ito IY. In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution. J Endodon 1999; 25(3): 167-171. [ Links ]
11. Lenet BJ, Komorowski R, Wu XY, Huang J, Grad H, Lawrence HP et al. Antimicrobial substantivity of bovine root denton exponed to different chlorhexidine delivery vehicles. J Endodon 2000; 26(11): 652-655. [ Links ]
12. Gomes BP, Souza SF, Ferraz CR, Teixeira FB, Zaia AA, Valdrighi L et al. Effectiveness of 2% chloerhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. Int Endod J 2003; 36: 267-275. [ Links ]
13. Gomes BP, Ferraz RC, Berber VB, Vianna ME, Teixera FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J 2001; 34: 424-428. [ Links ]
14. White RR, Hays GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endodon 1997; 23(4): 229-321. [ Links ]
15. White RR, Lusk SS. Effect to concentration and time on substantivity of a chlorhexidine endodontic irrigant. J Den Res 1998; 77: 271. [ Links ]
16. Weber CD, McClanahan SB, Millar GA, Diener-West M, Jonson JD. The effect of passive ultrasonic activation of 2% chlorhexidine or 5.25% sodium hypochlorite irrigan ton residual antimicrobial activity in root canals. J Endodon 2003; 29(9): 562-564. [ Links ]
17. Evans MD, Baumgarther JC, Khemaleelakul S, Xia T. Efficacy of calcium hydroxide: Chlorhexidine paste as an Intracanal medication in bovine dentin. J Endodon 2003; 29(5): 338-9. [ Links ]
18. Siqueira JF, Batista MM, Fraga RC, de Uzeda M. Antibacterial effects of endodontic irrigants on bleck-pigmented gramnegative anaerobes and facultative bacterial. J Endodon 1998; 24(6): 414-416. [ Links ]
19. Figuereido BP, Randi FC, Devora FG, Luiz PR, Zaia AA, Batista FT et al. Microbial susceptibility to calcium hydroxide pastes and their vehicles. J Endodon 2002; 28(11): 758-761. [ Links ]
20. Siqueira JF, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J 1999; 32: 361-369. [ Links ]
21. Estrela C, Rodríguez EC, Luschke BL, Djalma PJ. Two methods to evaluate the antimicrobial action of calcium hydroxide paste. J Endodon 2001; 27(12): 720-23. [ Links ]
22. Barthel CR, Levin LG, Reisner HM, Trope M. TNF-α release in monocytes after exposure to calcium hydroxide treated Escherichia coli LPS. Int Endod J 1997; 30: 155-159. [ Links ]
23. Olsen MH, Difiore PM, Dixit SN, Veis A. The effects of calcium hydroxide inhibition on LPS induced release of IL-1β from human monocytes in whole blood. J Endodon 1999; 25: 289. [ Links ]
24. Nelson-Filho P, Leonardo MR, Becerra SL, Assed S. Radiographic evaluation of the effect of endotoxin (LPS) plus calcium hydroxide on apical and periapical tissues of dogs. J Endodon 2002; 28(10): 694- 696. [ Links ]
25. Safavi KE, Spángberg LS, Langeland K. Root canal dentinal tubule disinfection. J Endodon 1990; 16(5): 207-210. [ Links ]
26. Hancock H, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2001; 91: 579-586. [ Links ]
27. Baker NE, Liewehr FR, Buxton TB, Joyce AP. Antibacterial efficacy of calcium hydroxide, iodine potassium iodide, betadine, and betadine scrub with and without surfactant against E. faecalis in vitro. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2004; 98: 359-64. [ Links ]
28. Waltimo TM, Ostavik D, Sirén EK, Haapasalo PP. In vitro susceptibility of Candida albicans to tour disinfectants and their combinations. Int Endod J 1999; 32: 421-429. [ Links ]
29. Lin Y, Mickel AK, Chogle S. Effectiveness of selected materials against Enterococcus faecalis: Part 3. The antibacterial effect of calcium hydroxide and Chlorhexidine on Enterococcus faecalis. J Endodon 2003; 29(9): 565-566. [ Links ]
30. Dameto FR, Randi FC, Figuereido BP, Zaia AA, Batista FT Souza-Filho FJ. In vitro of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2005; 99: 768-72. [ Links ]
31. Estrela C, Holland R, Bernabé PF, Souza V, Estrela CR. Antimicrobial potential of medicaments used in healing process in dogs’ teeth with apical periodontitis. Braz Dent J 2004; 15(3): 181-185. [ Links ]
32. Sirén EK, Haapasalo MP, Waltimo TM, Ostavik D. In vitro antibacterial effect of calcium hydroxide combined with chlorhexidine or iodine potassium iodide on Enterococcus faecalis. Eur J Oral Sci 2004; 112: 326-331. [ Links ]
33. Barbosa CA, Gonçalves, RB, Siqueira JF, De Uzeda M. Evaluation on the antibacterial activities of calcium hydroxide, chlorhexidine, and camphorated paramonochlorophenol as intracanal medicament. A clinical and laboratory study. J Endodon 1997; 23(5): 297-300. [ Links ]
34. Basrani B, Tjäderhance L, Santos JM, Pascon E, Grad H, Lawrence HP et al. Efficacy of chlorhexidine and calcium hydroxide containing medicaments against Enterococcus faecalis in vitro. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 2003; 96: 618-624. [ Links ]
35. Peters LB, van Winkelhoff AJ, Buijs JF, Wesselink PR. Effects of instrumentation, irrigation y dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. Int Endod J 2002; 35: 13-21. [ Links ]
36. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root filled teeth with apical periodontitis, Int Endod J 1998; 31: 1-8. [ Links ]
37. Sukawat C, Srisuwan T. A comparison of the antimicrobial efficacy of three calcium hydroxide formulations on human dentin infected with Enterococcus faecalis. J Endodon 2002; 28(2): 102-104. [ Links ]
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