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Revista mexicana de ciencias agrícolas
versão impressa ISSN 2007-0934
Resumo
FLORES GRACIA, Juan; MADRID GONZALEZ, Manuel e NODAL MORENO, Sergio Antonio. Use of Bacillus sp. as a resistance inducer in prickly pear against ‘scaly rot’. Rev. Mex. Cienc. Agríc [online]. 2023, vol.14, n.1, pp.25-37. Epub 19-Jun-2023. ISSN 2007-0934. https://doi.org/10.29312/remexca.v14i1.2898.
‘Scaly rot’ is one of the most important diseases in prickly pear, for this reason the objective of the present work was to isolate and identify fungi associated with ‘scaly rot’ and evaluate the ability of bacteria of the genus Bacillus sp. isolated to avoid the disease of ‘scaly rot’ in prickly pear [Opuntia ficus-indica (L.) Mill.]. Ten bacteria of the genus Bacillus sp. were analyzed in in vitro antagonism tests against the pathogen Neoscytalidium hyalinum, which was identified as the causative agent of ‘scaly rot’. The results of this study demonstrated that all isolates of Bacillus sp. significantly decreased (p≤ 0.05) the radius of the pathogen in the in vitro test, with isolates G11, G21 and G31 standing out. In the in vivo resistance induction tests, isolates G11, G21 and G31 significantly decreased (p≤ 0.05) the diameter of the lesions caused by Neoscytalidium hyalinum. The identification of the species used was carried out through a phylogenetic analysis of the sequences obtained of the regions 16s rRNA (bacteria) and ITS1-5.8s-ITS2 (fungi). Bacterial isolates G11, G21 and G31 were identified as Bacillus amyloliquefaciens and all fungi belonging to the genus Neoscytalidium sp. were identified as N. hyalinum.
Palavras-chave : Bacillus amyloliquefaciens; Neoscytalidium hyalinum; Opuntia ficus-indica (L.) Mill.; antagonism; resistance induction; scaly rot..