Resumo

CEBALLOS CEBALLOS, Augusto Gil; CERNA CHAVEZ, Ernesto; OCHOA FUENTES, Yisa María  e  RODRIGUEZ PAGAZA, Yolanda. New haplotypes of Diaphorina citri, vector of Candidatus Liberibacter in citrus areas of Mexico. Rev. Mex. Cienc. Agríc [online]. 2022, vol.13, n.8, pp.1387-1397.  Epub 19-Jun-2023. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v13i8.2882.

New haplotypes of Diaphorina citri, also known as the Asian citrus psyllid, were identified, called DcitACC-1, DcitACC-2, and DcitACC-3. The studies were based on the amplification of DNA of the mitochondrial COI gene and individuals from different citrus areas of the country were used, in some producing areas of the country no samplings have been carried out before, specifically in the municipalities of Acatlán de Pérez, Oaxaca, Misantla and Tantoyuca, Veracruz, and Huejutla, Hidalgo , so adult insects were collected without distinction of gender. The number of individuals collected from each site depended on the availability of insects at the site. A total of 60 individuals were collected. DNA amplification was performed with the specific primers DCITRI COI-L and DCITRI COI-R, the product of the PCR reaction was sequenced at the Institute for Scientific and Technological Research of San Luis Potosí (IPICYT, for its acronym in Spanish). The sequences obtained were compared with those reported in Genbank and it was determined that there is a matrix line corresponding to haplotypes Dcit-01 and Dcit-04 with identification numbers FJ190300 and FJ190 306 (Boykin, 2007). Twenty-two sequences were obtained, which were analyzed with the Oligo analyzer and Clustal Omega programs and 11 sequences equal to haplotypes Dcit-01 and Dcit-04 were identified. The results showed 13 sequences with differences in three specific nucleotides: 61, 253 and 636, which are reported in this work as new haplotypes.

Palavras-chave : Diaphorina citri; haplotypes; psyllid.

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