Resumo

HERNANDEZ, Prisciliano et al. Determination of Glucose by Flow Injection Analysis with Amperometric Detection at Fe(III)-(tris(3,5-dimethyl-1-pyrazolyl)borate)₂ Modified Electrodes. J. Mex. Chem. Soc [online]. 2011, vol.55, n.2, pp.120-125. ISSN 1870-249X.

A modified screen printed electrode with [Fe(tris(3,5-dimethyl-1-pyrazolyl)borate)₂]⁺[FeCl₄]^-, installed as part of a flow injection system, was used as an amperometric detector to determine hydrogen peroxide and glucose after on-line reaction with glucose oxidase. The influence of experimental variables on sensitivity, such as the flow rate, injection volume and reaction coil length, was evaluated using a two-level factorial experimental design. Under optimal conditions, detection limits of 15 μM and 0.5 mM for H₂O₂ and glucose, respectively, were obtained. A sampling rate of 60 determinations h^-1 was achieved; the relative standard deviation of analytical measurements was <5.0%. The method was validated by comparing the obtained results with those provided by the enzymatic spectrophoto-metric method; no significant differences were observed (p < 0.05).

Palavras-chave : Glucose; Hydrogen peroxide; Flow injection system.

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