Objective

The objective was to evaluate the antimicrobial effect of the OPW in concentration of 1% and 2.5% of NaCl against E. faecalis isolated from root canal.

Methodology

E. faecalis samples isolated from patients root canals retreatment with an apical lesion and diagnosis of chronic apical periodontitis were used. The samples were incubated in anaerobic conditions in CDC blood agar medium until their use. The serial CDC technique was used to evaluate the root canal irrigant. Eighty six samples were divided in 4 experimental groups of OPW (1% and 2.5%), with an irrigation scheme of 9 mL of OPW + 1 mL of inoculate and with 5 minutes of exposition to the irrigant. The incubation times for all samples were of 24 and 48 hours. In the positive control, 5.25% NaOCl was used and in the negative control, distilled water.

Results

The OPW was effective in concentrations of 1% and 2.5% NaCl, obtaining an average of 28 CFU and 2 CFU for 1% and 2.5% OPW, respectively.

Conclusions

The OPW has an antimicrobial effect in the concentrations used of NaCl (1% and 2.5%). Nevertheless, it is necessary to study the efficacy of this irrigant on the bacterial biofilm and its biocompatibility in order to propose it, as a root canal irrigant.

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