Resumen

GONZALEZ, Dolores. Optimization to the SCAR (Sequence Characterized Amplified Region) favors the isolation of polymorphic loci for phylogenetic studies in closely related taxa. Rev. Mex. Biodiv. [online]. 2010, vol.81, n.1, pp.183-185. ISSN 2007-8706.

DNA sequence data have made a tremendous contribution to plant phylogenetics. Nonetheless, a notable shortcoming of several loci has been the poor phylogenetic resolution of relationships among closely related species. In this study a modification to the SCAR method is proposed that consists of: a) digestion of polymorphic RAPD fragments instead of cloning them, b) sequencing of the digested fragments, and c) alignment of sequences for designing specific primers. This simple approach allowed us to find variable DNA loci suitable for phylogenetic analysis at lower taxonomic ranks.

Palabras llave : molecular phylogeny; molecular markers; plant systematics; restriction enzymes; Digested-SCAR; RAPD.

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