Resumen

GUERRERO-VALENCIA, F.A. et al. Micropropagation of amazon lily (Eucharis grandiflora Planch. & Linden) through direct organogenesis. Polibotánica [online]. 2021, n.51, pp.141-153.  Epub 06-Mayo-2021. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.51.9.

Amazon lily (Eucharis grandiflora Planch. & Linden) is a geophyte plant for ornamental use and it is propagated through the division of adventitious shoots. However, this method is limited, because a bulb produces only two or three offspring after one year of cultivation, so the objective of this research was to develop a methodology that allows the micropropagation of this species through direct organogenesis and thus increase the number of new seedlings in commercial systems. During the in vitro establishment, there were used 0.5 X 1.0 cm explants conformed by a portion of the scale and basal disk and they were cultivated in Murashige & Skoog medium (MS) supplemented with 2.0 ml·L-1 of Plant Preservative Mixture (PPM™). For the induction and multiplication of adventitious shoots, the effect of 6-benzyl-aminopurine (BAP) was evaluated in concentrations of 0.0, 0.1, 0.3, 1.0 and 3.0 mg·L-1 and the number of new shoots every week was determined. The shoots obtained were subcultured in a medium without regulators to promote their development, and the sucrose content was evaluated in concentrations of 30 to 70 g·L-1. For rooting, indol-3-acetic acid (AIA), indol-3-butyric acid (AIB), and α-naphthaleneacetic acid (ANA) were used, in concentrations of 0.3 and 1.0 mg·L-1. Incubation conditions were a photoperiod of 16 light hours and 8 dark hours, at a temperature of 25 ± 2 ºC and a light intensity of 50 µmol·m-2·s-1. The rooted shoots were acclimatized in pots with peat moss and were kept in a glass greenhouse with 50% shade. In the establishment, contamination of 30% was obtained. During multiplication, the use of 3.0 mg·L-1 BAP produced a maximum of 3.8 shoots per explant 35 days after culture. The best development of shoots was obtained with 50 g·L-1 of sucrose. In rooting, AIA, and AIB (0.3 mg·L-1) produced a greater root length. During acclimatization, 100% survival was obtained 50 days after transplanting and acclimatization.

Palabras llave : Amaryllidaceae; biotechnology; propagation; bulb.

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