Resumen

QUISPE-APAZA, Cinthia Sheila et al. Genetic diversity of Hemileia vastatrix of two coffee producing areas in Peru. Rev. mex. fitopatol [online]. 2017, vol.35, n.3, pp.418-436. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1612-7.

The genetic diversity of the coffee pathogen Hemileia vastatrix was analyzed by sequencing the internal transcribed spacer (ITS) of ribosomal DNA. The analysis was carried out using samples of rust pustules from 12 properties of two coffee areas in Peru, Quillabamba (Cusco) and Villa Rica (Pasco). Haplotype diversity indexes were found and the similarities among the populations analyzed were determined using a haplotype network. From the analysis, it was determined that both producing regions presented high values of genetic diversity, but Quillabamaba harbored the highest haplotype diversity of H. vastatrix (Hd = 0.977 +/- 0.012). In addition, the haplotype network showed the structure of H. vastatrix populations for these two areas, which behave as a large undifferentiated population with the presence of ancestral haplotypes from which new variants of the fungus were generated. We also analyzed the sequences of the ITS region of H. vastatrix collected in Peru and those reported in the GenBank, but we did not find any distribution of the populations by geographic region; it was also suggested that the populations of Peruvian rust presented haplotypes similar to those from Colombia and races II and XXII.

Palabras llave : Yellow rust; ITS; Coffea spp; haplotypes.

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